Pendin Diana
Researcher
c/o Complesso Biologico Interdipartimentale
A. Vallisneri
Viale Giuseppe Colombo 3
35121 Padova
Tel 049-8276096
Fax 049-8276040
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My research focuses on understanding the role of Ca2+ homeostasis dysregulation in the development of neurodegenerative disorders. In particular, we employ Ca2+ imaging techniques to study Ca2+ homeostasis in models of neurodegenerative diseases such as Alzheimer’s disease and Hereditary Spastic Paraplegia. Our interest is not restricted to cytosolic Ca2+ dynamics, but covers also organelles Ca2+ regulation. We recently developed several new tools to study in vivo Ca2+ dynamics, including viral vectors and transgenic animals (mice and Drosophila) expressing FRET-based Ca2+ probes.
The current topic of my research concerns the study of the molecular basis of the pathogenesis of the neurodegenerative disease Hereditary Spastic Paraplegia, using Drosophila melanogaster as an animal model. In particular, we are interested in understanding how ER morphology alterations affect neuronal function in HSP models.
http://www.biomed.unipd.it/research-areas/cell-signaling/ca2-and-camp-signalling-in-physiology-and-pathology/people/
Representative publications
Microtubules Stabilization by Mutant Spastin Affects ER Morphology and Ca2+ Handling. Vajente N, Norante R, Redolfi N, Daga A, Pizzo P, Pendin D. Front Physiol. 2019 Dec 20;10:1544.
Dynamic constriction and fission of endoplasmic reticulum membranes by reticulon. Espadas J*, Pendin D*, Bocanegra R, Escalada A, Misticoni G, Trevisan T, Velasco Del Olmo A, Montagna A, Bova S, Ibarra B, Kuzmin PI, Bashkirov PV, Shnyrova AV, Frolov VA, Daga A. Nat Commun. 2019 Nov 22;10(1):5327. *Equal contribution.
A Synthetic Fluorescent Mitochondria-Targeted Sensor for Ratiometric Imaging of Calcium in Live Cells. , Norante R, De Nadai A, Gherardi G, Vajente N, Basso E, Kaludercic N, Mammucari C, Paradisi C, Pozzan T, Mattarei A. . 2019 May 27.
Manipulation of Mitochondria Dynamics Reveals Separate Roles for Form and Function in Mitochondria Distribution. Trevisan T*, Pendin D*, Montagna A, Bova S, Ghelli AM, Daga A. Cell Rep. 2018 May 8;23(6):1742-1753. *Equal contribution.
GTP-dependent packing of a three-helix bundle is required for atlastin-mediated fusion. Pendin D, Tosetto J, Moss TJ, Andreazza C, Moro S, McNew JA, Daga A. Proc Natl Acad Sci U S A. 2011 Sep 27;108(39):16283-8.
Homotypic fusion of ER membranes requires the dynamin-like GTPase atlastin. Orso G*, Pendin D*, Liu S, Tosetto J, Moss TJ, Faust JE, Micaroni M, Egorova A, Martinuzzi A, McNew JA, Daga A. Nature. 2009 Aug 20;460(7258):978-83. *Equal contribution.