Borgese Nica
Full Professor
Via Luigi Vanvitelli 32
20129 Milano
Tel 02-50316947
Fax 02-7490574
n.borgese @ in.cnr.it
Protein trafficking into and out of the Endoplasmic Reticulum
Research summary
The research in my laboratory is focussed on the biology of the Endoplasmic Reticulum in neuronal as well as non-neuronal cells. Specifically, we are interested in the mechanisms of insertion of transmembrane proteins into the Endoplasmic Reticulum, in the effect of membrane proteins on the architecture and dimensions of this organelle and in the modes of export of membrane proteins from the Endoplasmic Reticulum down the secretory pathway. In our studies, we use an interdisciplinary approach, which combines biochemistry with live cell imaging, and, recently, biophysical methodology. In recent years, we have applied our expertise to the analysis of the pathogenic mechanism of a mutant Endoplasmic Reticulum protein linked to Amyotrophic Lateral Sclerosis.
Representative publications
Giordano F., Saheki Y., Idevall-Hagren Y., Colombo S.F.., Pirruccello M., Milosevic I., Gracheva E.O., Bagriantsev S.N., Borgese N., De Camilli P. (2013)
PI(4,5)P2 dependent and Ca2+-regulated ER-Plasma Membrane Interactions mediated by the Extended-Synaptotagmins. Cell 153: 1494-1509
Maiuolo S., Bulotta S., Verderio C., Benfante R., Borgese N. (2011)
Selective activation of the transcription factor ATF6 mediates endoplasmic reticulum proliferation triggered by a membrane protein. Proc Natl Acad Sci U S A. 108:7832-7837.
Borgese N., Righi M. (2010)
Remote Origins of Tail-Anchored Proteins. Traffic 11: 877-885
Fasana E., Fossati M., Ruggiano A., Brambillasca S., Hoogenraad C., Navone F., Francolini M., Borgese N. (2010)
A VAPB mutant linked to amyotrophic lateral sclerosis generates a novel form of organized smooth endoplasmic reticulum. FASEB J. 24: 1419-1430
Colombo S.F., Longhi, R., Borgese, N. (2009)
The Role Of Cytosolic Proteins In The Insertion Of Tail-Anchored Proteins Into Phospholipid Bilayers. J. Cell Sci. 122: 2383-92
Ronchi P., Colombo S., Francolini M., Borgese N. (2008)
Transmembrane Domain-Dependent Partitioning of Membrane Proteins within the Endoplasmic Reticulum. J. Cell Biol. 181: 105-118